TABLE 2.
Effects of dorY, dorR, and ccoP mutations on dorC::lacZ expression
| Strain | β-Galactosidase activity after
growtha
|
|
|---|---|---|
| Photoheterotrophic | Photoheterotrophic + DMSO | |
| 2.4.1T (wild type) | 12 ± 7 | 2,683 ± 297 |
| NM16 (DorR) | 17 ± 5 | 39 ± 5 |
| NM22 (DorY) | 19 ± 7 | 23 ± 14 |
| CCOP1 (CcoP) | 567 ± 70 | 3,813 ± 569 |
| NM23 (DorY CcoP) | 12 ± 10 | 783 ± 60 |
| NM24 (DorR CcoP) | 2 ± 1 | 7 ± 3 |
a
Cultures were grown to mid-log phase under photosynthetic conditions at a light intensity of 10 W m−2 in the presence or absence of 60 mM DMSO. Cell extracts were assayed for β-galactosidase activity. Units of activity are micromoles of o-nitrophenol (ONP) formed minute−1 milligram of protein−1. Values represent the mean values ± the standard deviations from triplicate assays of at least three independent growths and are corrected for activity from the vector alone under the same conditions (pML5, <35 μmol of ONP formed min−1 mg of protein−1).