TABLE 3.
Effects of a dorC mutation on dorC::lacZ expression
| Strain | β-galactosidase activity after
growtha:
|
|||
|---|---|---|---|---|
| Aerobic | Aerobic + DMSO | Photohetero-trophic | Photoheterotrophic + DMSO | |
| 2.4.1T (wild type) | 1 ± 1 | 4 ± 3 | 12 ± 7 | 2,683 ± 297 |
| NM19 (DorA) | 4 ± 3 | 30 ± 4 | 932 ± 53 | 1,048 ± 172 |
a
Cultures were grown to mid-log phase under the conditions indicated and assayed for β-galactosidase activity. Photosynthetic growth occurred at a light intensity of 10 W m−2. Units of activity are micromoles of o-nitrophenol (ONP) formed minute−1 milligram of protein−1. Values represent the mean values ± the standard deviations from triplicate assays of at least three independent growths and are corrected for activity from the vector alone under the same conditions (pML5, <35 μmol of ONP formed min−1 mg of protein−1).