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. 2024 Jan 2;15:26. doi: 10.1038/s41467-023-44407-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Fluorescence intensity time traces acquired at different concentrations of Alexa 647 in phosphate buffer solution (PBS). b Retrieved autocorrelation functions from selected time traces in Panel a. Autocorrelation functions at 1 and 6 nM are multiplied by 3 for clarity. c Number of molecules in metalens detection volume directly retrieved from FCS correlation functions and background intensity data. Error bars denote standard deviation of FCS experiment. d Normalized FCS correlation functions of Alexa 647 in the presence and absence of glycerol. Diffusion time in glycerol 60 vv% solution rises by approximately 14× owing to viscosity increase. e Fluorescence decay data of Alexa 647 in the absence and presence of glycerol 60 vv%.