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. 2024 Jan 2;15:26. doi: 10.1038/s41467-023-44407-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Set of lenses employed for comparative study with indicated size dimensions: metalens, two single-element aspheric lenses of NA = 0.18 and NA = 0.54, and achromatic objective lenses of NA between 0.15 and 1.2. b Alexa 647 single molecule brightness (CRM) detected by metalens and aspheric lenses. As FCS correlation is not detectable by aspheric lenses, single molecule brightness is considered to equal zero. c Alexa 647 single molecule brightness detected by metalens and achromatic objective lenses. d Alexa 647 molecule diffusion time (τ_d) detected by metalens and achromatic objective lenses. e Confocal effective volume (V_eff) observed by metalens and achromatic objective lenses. Error bars denote standard deviation of FCS experiment.