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. 2024 Jan 2;10:2. doi: 10.1038/s41523-023-00605-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Following the selection of qualified ER, PR, and HER2 CyCIF antibodies, the expression of selected antibodies was evaluated at a single-cell level in 567 HER2+ invasive breast cancer samples from 189 patients, and t-Distributed Stochastic Neighbor Embedding (t-SNE) is shown as a distribution of all single cells. a Selected markers were used to plot single cells. b t-SNE in a is shown colored by patient ID. c Visualization of markers within t-SNE plots.