Table 2.
Antibody staining panels used for BC03 TMA.
| Cycle # | BC03_A (PR/Ki67) | BC03_B (ER/p53) | BC03_C (AR/p53/Ki67) | BC03_D (HER2) | ||||
|---|---|---|---|---|---|---|---|---|
| Background | Hoechst1 | Hoechst1 | Hoechst1 | Hoechst1 | Hoechst1 | Hoechst1 | Hoechst1 | Hoechst1 |
| FITC_1 | A488 | FITC_1 | A488 | FITC_1 | A488 | FITC_1 | A488 | |
| Cy3_1 | A555 | Cy3_1 | A555 | Cy3_1 | A555 | Cy3_1 | A555 | |
| Cy5_1 | A647 | Cy5_1 | A647 | Cy5_1 | A647 | Cy5_1 | A647 | |
| 2 | Hoechst2 | Hoechst2 | Hoechst2 | Hoechst2 | Hoechst2 | Hoechst2 | Hoechst2 | Hoechst2 |
| FITC_2 | HER2 (TF-MA5-14509) | FITC_2 | ER (ab16660) | FITC_2 | p53 (ab32389) | FITC_2 | HER2 (TF-MA5-14509) | |
| Cy3_2 | 14-3-3 (sc-629-G) | Cy3_2 | 14-3-3 (sc-629-G) | Cy3_2 | 14-3-3 (sc-629-G) | Cy3_2 | 14-3-3 (sc-629-G) | |
| Cy5_2 | PR (Dako-M3569) | Cy5_2 | p53 (Dako_M7001) | Cy5_2 | AR (TF-MA5-13426) | Cy5_2 | p53 (ab154036) | |
| 3 | Hoechst3 | Hoechst3 | Hoechst3 | Hoechst3 | Hoechst3 | Hoechst3 | Hoechst3 | Hoechst3 |
| FITC_3 | PR-488 (ab199244) | FITC_3 | PR-488 (ab199244) | FITC_3 | p53-488 (CS5429) | FITC_3 | HER2-488 (RD-FAB9589G) | |
| Cy3_3 | PR-PE (CS23353) | Cy3_3 | ER-PE (CS74244) | Cy3_3 | AR-555 (CS8956) | Cy3_3 | HER2-PE (CS98710) | |
| Cy5_3 | PR-647 (ab199455) | Cy5_3 | PR-660 (EB50-9764-80) | Cy5_3 | AR-647 (AB194195) | Cy5_3 | HER2-647 (BL324412) | |
| 4 | Hoechst4 | Hoechst4 | Hoechst4 | Hoechst4 | Hoechst4 | Hoechst4 | Hoechst4 | Hoechst4 |
| FITC_4 | PR-488 (ab199244) | FITC_4 | Ki67-488 (CS11882) | FITC_4 | PR-488 (ab199244) | FITC_4 | Ki67-488 (CS11882) | |
| Cy3_4 | Ki67-570 (EB41-5699-82) | Cy3_4 | ER-568 (ab207261) | Cy3_4 | CK-570 (EB41-9003-82) | Cy3_4 | CK-570 (EB41-9003-82) | |
| Cy5_4 | PR-660 (EB50-9764-80) | Cy5_4 | HER2-647 (RD-FAB1129R) | Cy5_4 | p53-647 (ab224942) | Cy5_4 | HER2-647 (ab225510) | |
| 5 | Hoechst5 | Hoechst5 | Hoechst5 | Hoechst5 | Hoechst5 | Hoechst5 | Hoechst5 | Hoechst5 |
| FITC_5 | Ki67-488 (CS11882) | FITC_5 | p53-488 (CS5429) | FITC_5 | Ki67-488 (CST11882) | FITC_5 | PR-488 (ab199244) | |
| Cy3_5 | ER-PE (CS74244) | Cy3_5 | CK-570 (EB41-9003-82) | Cy3_5 | Ki67-570 (EB41-5699-82) | Cy3_5 | CK-555 (CS3478) | |
| Cy5_5 | Ki67-647 (CS12075) | Cy5_5 | p53-647 (ab224942) | Cy5_5 | Ki67-647 (BL350509) | Cy5_5 | HER2-647 (RD-FAB1129R) | |
| 6 | Hoechst6 | Hoechst6 | Hoechst6 | Hoechst6 | Hoechst6 | Hoechst6 | Hoechst6 | Hoechst6 |
| FITC_6 | p53-488 (CS5429) | FITC_6 | HER2-488 (RD-FAB9589G) | FITC_6 | HER2-488 (RD-FAB9589G) | FITC_6 | p53-488 (CS5429) | |
| Cy3_6 | ER-568 (ab207261) | Cy3_6 | PR-PE (CS23353) | Cy3_6 | HER2-PE (CS98710) | Cy3_6 | ER-PE (CS74244) | |
| Cy5_6 | ER-647 (ab205851) | Cy5_6 | PR-647 (ab199455) | Cy5_6 | HER2-647 (BL324412) | Cy5_6 | AR-647 (AB194195) | |