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. 2024 Jan 2;14:218. doi: 10.1038/s41598-023-50763-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differential activity of NICD heterodimers on various promoters. 293T cells were co-transfected with luciferase reporters containing either the Hes1 (top), Hes4 (middle), or Hes5 (bottom) promoters and either WT NICDs, N2ICD-KK, N4ICD-EE, or a combination of KK and EE NICDs. Hes1 data represents the average +/− SE of five experiments while Hes4 and Hes5 data represents the average +/− SE of four experiments. Statistical significance was determined through a student’s two-tailed t test, assuming equal variances, where *** is p < 0.001, ** is p < 0.01, and * is p < 0.05.