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. 2024 Jan 2;15:175. doi: 10.1038/s41467-023-44157-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Free phage titers of Paride after infecting deep-dormant cultures of different clinical isolates of P. aeruginosa for 48 h (dashed line: inoculum). b P. aeruginosa clinical isolate CI249 subcultured for 48 h was treated with antibiotics or Paride (MOI ≈ 0.01) and viable CFU/ml as well as free phages were recorded over time. Due to lack of robust growth in M9Glc this experiment was performed in M9Rich (see Fig. S4a for a control experiment with the P. aeruginosa PAO1 Δpel Δpsl in this medium). Data points and error bars show average and standard error of the mean of three independent experiments. Limits of detection are 2 log10 CFU/mL for viable cells, 3.6 log10 PFU/mL for free phages. c, d Burst size and latency of ancestral Paride as well as two evolved clones passaged on deep-dormant cultures were determined by one-step growth experiments (see also Fig. S4b, c). Data bars represent the average of two (non-growing hosts) or six (growing hosts) independent experiments and all individual data points are shown. Source data are provided as a Source data file.