Figure 4.

Minos insertions having a w¹¹¹⁸ control affect sleep latency and exhibit pleiotropic effects on some sleep phenotypes. The plots show mean sleep phenotypes ± SEM in Minos insertion lines contrasted with the sleep phenotypes of the w¹¹¹⁸ isogenic control. Light purple bars indicate female data, with the w¹¹¹⁸ control shown in dark purple. Light blue bars indicate male data, with the w¹¹¹⁸ control shown in dark blue. (a) Sleep latency. (b) Night avg. bout length. (c) Day sleep duration. (d) Day avg. bout length. Asterisks show the level of significance: **** P ≤ 0.0001; *** 0.0001 < P ≤ 0.001; ** 0.001 < P ≤ 0.0031. A pound sign (#) shows those mutants that are also significantly different from the overall mean of all mutant data combined.