Fig. 5. Scavenging ROS reverses lipodystrophy in Med20-AKO mice.

a Chow-fed male Med20^f/f and Med20-AKO mice (6-week-old, n = 6) were daily gavaged with vehicle or BHA (10 mg/ml in corn oil) for 8 weeks. b Body weights were monitored every week for 8 weeks. Glucose (c) and insulin (d) tolerance tests were performed on week 7 and 8, respectively. e Body composition was analyzed on week 8. f On week 8, mice were euthanized. Representative images of iWAT and gWAT were shown. g Plasma and liver triglyceride levels were measured. h Plasma H₂O₂ level was measured. i iWAT was subjected to H&E and immunostaining using anti-Perilipin and Galectin3 antibodies as in Fig. 4j. Each value represents mean ± s.e.m. of 6 mice. j A schematic summary of the key findings in this figure. Scale bars are as indicated. Statistical analysis was performed using two-sided unpaired Student’s t-tests.