Fig. 6. Glutathione reverses stavudine-induced partial lipodystrophy in mice.

a–c Primary SVFs from WT mice were differentiated into mature adipocytes. On day 0, cells were treated with stavudine (1 mM) with or without GSH (1 mM). On day 6, cells were harvested for imaging under bright field (a) and quantification of cell numbers (b). For b, each value represents mean ± s.e.m. of 3 samples. On day 4, cells were treated with CellROX (2.5 μM) for 30 min and harvested for ROS quantification (c). For c, each value represents mean ± s.e.m. of 70 samples. d–k C57BL6 mice were subjected to HFD for 4-weeks, and then treated with stavudine (5 mg/mL in water) with daily gavage of GSH (100 mg/kg) for 9 more weeks. Glucose (e) and insulin (f) tolerance tests were performed on week 11 and 12, respectively. On week 13, mice were euthanized. Percentage of iWAT/body weight was plotted in (g). Representative images were shown in (h). i H&E and immunostaining of iWAT using anti-Perilipin and Galectin3 antibodies were performed as in Fig. 4j. j Liver triglyceride levels were measured. k Plasma H₂O₂ levels were measured. l A schematic summary of the key findings of the figure. Each value represents mean ± s.e.m. of 6 mice. Statistical analysis was performed using two-sided unpaired Student’s t-tests.