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. 2024 Jan 2;15:186. doi: 10.1038/s41467-023-44559-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a aP2-ApoL6 TG design (left upper), RT-qPCR for transgene expression (left lower, n = 3 mice, ***p < 0.001 compared to WT), immunoblotting of transgene expression in different tissues and ApoL6 protein levels in WAT (right). b BW in chow (n = 6 mice per group, **p = 0.0057 and 0.0027, ***p < 0.00, two-way ANOVA test). c Tissue weights of 24 wk-old mice (left, n = 6, *p = 0.03, ***p < 0.001, multiple t test). H&E staining (bar = 100 μm) and quantification of cell sizes of WAT (right two panels). d Serum FFA (fed and fasted, n = 6, *p = 0.0117, multiple t test), TAG (n = 6, *p = 0.0224), and cholesterol levels in fed condition (n = 6, **p = 0.0034, *p = 0.049). Data represent mean ± SD. e BW during HFD (n = 6, **p = 0.006, *p = 0.0104, ***p < 0.001) and EchoMRI after HFD (n = 6, **p = 0.007 and 0.005). f Tissue image and tissue weight after 12 wks of HFD feeding (n = 6, ***p < 0.001). Data represent mean ± SD. g Serum FFA, TAG (n = 6, *p = 0.0214) and cholesterol levels after HFD feeding in the fed condition (n = 6 ***p < 0.001, **p = 0.0239). h GTT (n = 6, ***p < 0.001) and ITT (n = 6, *p = 0.0162 and 0.0319, ***p < 0.001, two-way ANOVA test) after HFD, Experiment was repeated twice. i RT-qPCR for inflammatory markers in WAT after HFD (n = 6, *p = 0.045, 0.032, **p = 0.0089, ***p < 0.001, multiple t test). j adipoQ-ApoL6 TG design. Immunoblotting using lysates of WAT from adipoQ-ApoL6 TG. BW (n = 5 mice per group, ***p < 0.001) and WAT mass measured by using EchoMRI (n = 6, ***p < 0.001) and tissue weights after 12 wks on HFD (n = 6, ***p < 0.001). k Image of tissues and whole mount staining of WAT with LipidTOX green (bar = 10 μm) after HFD feeding. l In vivo lipolysis assay: Serum glycerol levels at different time points after mice were injected with isoproterenol at 10 mg/kg BW (n = 6, ***p < 0.001, two-way ANOVA test). Experiments were repeated twice. m FFA and glycerol release from dispersed adipocytes isolated from WAT of aP2-TG (n = 4, *p = 0.005 and 0.006, ***p < 0.001) (left two panel). FFA and glycerol release from dispersed adipocytes isolated from WAT of adipoQ-ApoL6 TG (n = 6. *p = 0.026, 0.021, **p = 0.0024 and 0.0023). Data represent mean ± SD. Experiments were repeated twice.