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. 2024 Jan 2;15:14. doi: 10.1038/s41467-023-44514-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Gene set enrichment analyses of HALLMARK gene sets comparing baseline (pre-BAT) to C4D1 (On-BAT) in responders (N = 6; red dots) and non-responders (N = 6;purple dots) or responders to non-responders on C4D1 (On-BAT) (green dots). B Enrichment plot of ALLOGRAFT_REJECTION comparing pre-BAT to On-BAT in responding patients. Top 30 transcripts based on ranked metric scores are shown. C Enrichment plot of ALLOGRAFT_REJECTION comparing responders to non-responders on C4D1. Top 30 transcripts based on ranked metric scores are shown. FDR adj p, p-value adjusted for false discovery rate. ES, enrichment score. NES, normalized enrichment score. This is a two-tailed test where there is a multiple comparison adjustment for the statistics derived from the test (NES, FDR) based on the number of gene sets tested, number of samples and by applying numerous permutations of the gene list supplied and labels assigned to the samples.