Fig. 3. GCA exacerbates adipose tissue inflammation in NCD-fed mice.

a Inflammatory cytokine gene expression levels in eWAT from NCD-fed WT mice treated with PBS or rGCA (n = 6). b F4/80 immunohistochemistry and flow analyses of macrophage in eWAT from NCD-fed WT mice treated with PBS or rGCA (n = 3). Scale bar, 250 µm. c QPCR analysis of the proinflammatory Th1 marker genes (Tbx21 and Ifng), Treg (Foxp3) and Th2 (Gata3) in SFV from NCD-fed WT mice treated with PBS or GCA (n = 6). d Flow cytometry analysis of CD4+Tbet+ Th1 cells and CD4+Foxp3+ Treg cells in eWAT from NCD-fed WT mice treated with PBS or GCA (n = 3). Data are presented as means ± SEM. n indicates the number of biologically independent samples examined. Statistical analysis was assessed by two-sided Student’s t test and significant differences were indicated with p values. Source data are provided as a Source Data File.