Table 6.

Primer sequences used for MTHFR (rs1801133) and (rs1801131) polymorphisms.

Mutation	Primer sequence	Size (bp)
MTHFR (rs1801133) c.677C>T	Common F (FO): CCCAGCCACTCACTGTTTTAGTTCAGGC	407
	Common R(RO): GGTGAGAGTGGGGTGGAGGGAGCTTAT
	R (C allele): CAAAGAAAAGCTGCGTGATGATGAAATAGG	273
	R (T allele): TTGAAGGAGAAGGTGTCTGCGGGCGT	190
MTHFR A1298C (rs1801131)	Common F (FO): 5ʹ-GAAGAAGTTTGCATGCTTGTGGTTG-3ʹ	593
	Common R(RO): 5ʹ-CAGGCAAGTCACCTGGGAGAGA-3ʹ
	R (A allele): 5ʹ-GGCAAAGAACGAAGACTTCAAAGACACATT-3ʹ	281
	R (C allele): 5ʹ-GAGGAGCTGACCAGTGATGC-3ʹ	361