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. 2024 Jan 2;15:27. doi: 10.1038/s41467-023-44301-z
Reference Provider Dilution
Primary antibodies
rabbit polyclonal anti-GFP #A-6455 ThermoFisher Sc. 1/500 or 1/10 000
mouse monoclonal anti-Flag #F1804 Sigma-Aldrich 1/500 or 1/10 000
mouse monoclonal anti-TH #MAB318 Merck Millipore 1/1000
mouse anti-CK1 alpha #sc-75582 Santa Cruz 1/1000
mouse monoclonal anti-beta-tubulin TUB2.1 Sigma-Aldrich 1/5000
Rabbit anti-GluN2A Clone A12W Merck Millipore 1/1000 (WB)
rabbit anti-GluN2A custom-made Agrobio 1/200 (IF)
rabbit anti-GluN2B custom-made Agrobio 1/200 (IF), 1/1000 (WB)
rabbit anti-D1R 17934-1-AP Proteintech 2 µg
rabbit anti-D2R 55084-1-AP Proteintech 2 µg
rabbit anti-NR2A NB300-105 Novus Biologicals 1/1000
rabbit anti-NR2B ab65783 Abcam 1/1000
Chicken anti-MAP2 ab5392 Abcam 1/5000
Mouse anti-synapsin 1 #106011 Synaptic System 1/1000
Mouse anti-PSD95 MA1-046 ThermoFisher Sc. 1/500 (IF), 1/1000 (WB)
Rabbit anti-DBH ab209487 Abcam 1/2000
Secondary antibodies:
goat anti-mouse alexa fluor 488 #A11001 ThermoFisher Sc. 1/1000
Donkey anti-mouse alexa fluor 647 #A31571 ThermoFisher Sc. 1/1000
goat anti-rabbit alexa fluor 647 #A21244 ThermoFisher Sc. 1/1000
Goat anti-chicken 488 #A11039 ThermoFisher Sc. 1/1000
anti-mouse H + L HRP #715-035-150 Jackson Immunoresearch 1/5000
goat-anti-rabbit IgG (H + L) highly cross-absorbed secondary antibody Alexa Fluor Plus 800 #A32735 ThermoFisher Sc. 1/5000
Alexa Fluor 790 AffiniPure Goat-anti-Rat IgG (light chain specific) #112-655-175 Jackson ImmunoResearch 1/1000
Alexa Fluor 790 AffiniPure Goat-anti-Rabbit IgG (light chain specific) #115-655-174 Jackson ImmunoResearch 1/1000
Quantum Dots:
F (ab’)2-Goat anti-Rabbit IgG-coupled Qdot655 #Q11422MP ThermoFisher Sc. 1/50000
F (ab’)2-Goat anti-Rabbit IgG-coupledQdot705 #Q11461MP ThermoFisher Sc. 1/50000