Reference | Provider | Dilution | |
---|---|---|---|
Primary antibodies | |||
rabbit polyclonal anti-GFP | #A-6455 | ThermoFisher Sc. | 1/500 or 1/10 000 |
mouse monoclonal anti-Flag | #F1804 | Sigma-Aldrich | 1/500 or 1/10 000 |
mouse monoclonal anti-TH | #MAB318 | Merck Millipore | 1/1000 |
mouse anti-CK1 alpha | #sc-75582 | Santa Cruz | 1/1000 |
mouse monoclonal anti-beta-tubulin | TUB2.1 | Sigma-Aldrich | 1/5000 |
Rabbit anti-GluN2A | Clone A12W | Merck Millipore | 1/1000 (WB) |
rabbit anti-GluN2A | custom-made | Agrobio | 1/200 (IF) |
rabbit anti-GluN2B | custom-made | Agrobio | 1/200 (IF), 1/1000 (WB) |
rabbit anti-D1R | 17934-1-AP | Proteintech | 2 µg |
rabbit anti-D2R | 55084-1-AP | Proteintech | 2 µg |
rabbit anti-NR2A | NB300-105 | Novus Biologicals | 1/1000 |
rabbit anti-NR2B | ab65783 | Abcam | 1/1000 |
Chicken anti-MAP2 | ab5392 | Abcam | 1/5000 |
Mouse anti-synapsin 1 | #106011 | Synaptic System | 1/1000 |
Mouse anti-PSD95 | MA1-046 | ThermoFisher Sc. | 1/500 (IF), 1/1000 (WB) |
Rabbit anti-DBH | ab209487 | Abcam | 1/2000 |
Secondary antibodies: | |||
goat anti-mouse alexa fluor 488 | #A11001 | ThermoFisher Sc. | 1/1000 |
Donkey anti-mouse alexa fluor 647 | #A31571 | ThermoFisher Sc. | 1/1000 |
goat anti-rabbit alexa fluor 647 | #A21244 | ThermoFisher Sc. | 1/1000 |
Goat anti-chicken 488 | #A11039 | ThermoFisher Sc. | 1/1000 |
anti-mouse H + L HRP | #715-035-150 | Jackson Immunoresearch | 1/5000 |
goat-anti-rabbit IgG (H + L) highly cross-absorbed secondary antibody Alexa Fluor Plus 800 | #A32735 | ThermoFisher Sc. | 1/5000 |
Alexa Fluor 790 AffiniPure Goat-anti-Rat IgG (light chain specific) | #112-655-175 | Jackson ImmunoResearch | 1/1000 |
Alexa Fluor 790 AffiniPure Goat-anti-Rabbit IgG (light chain specific) | #115-655-174 | Jackson ImmunoResearch | 1/1000 |
Quantum Dots: | |||
F (ab’)2-Goat anti-Rabbit IgG-coupled Qdot655 | #Q11422MP | ThermoFisher Sc. | 1/50000 |
F (ab’)2-Goat anti-Rabbit IgG-coupledQdot705 | #Q11461MP | ThermoFisher Sc. | 1/50000 |