FIG. 1.

Western immunoblotting assay for the expression of ChvE protein. Cells were incubated on induction medium with different monosaccharides and other additives. (A) Protein profiles of different A. tumefaciens cultures as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (B) Western immunoblotting. Lanes: 1, C58ΔvirA(pGP159) grown in GIB plus 10 mM arabinose; 2, C58ΔvirA(pGP159) grown in GIB plus 10 mM glucose; 3, C58ΔvirA(pGP159, pWT121) grown in GIB plus 10 mM glucose; 4, C58ΔvirA(pGP159, pWT121) grown in GIB plus 10 mM glucose plus 1 mM IPTG; 5, At11058(pGP159, pWT122) grown in GIB plus 10 mM glucose; 6, At11058(pGP159) grown in GIB plus 10 mM arabinose. The numbers on the left are molecular masses.