TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Agrobacterium strains | ||
| C58 | Wild-type nopaline strain | 25 |
| A6 | Wild-type octopine strain | 25 |
| KU12 | Wild-type octopine strain | 20 |
| A136 | C58; Ti plasmid cured | 25 |
| A348 | A136(pTiA6) | 13 |
| A136MX1 | A136 chvE::Tn5 | 18 |
| C58ΔvirA | C58(ΔvirA) | 20 |
| At11058 | C58 chvE::Tn5 virA::Ω | T. Charles |
| A136gbpR | A136 gbpR | This work |
| C58ΔvirAgbpR | C58(ΔvirA) gbpR | This work |
| E. coli strains | ||
| DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 φ80d lacZ ΔM15 | Bethesda Research Laboratories |
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15] | U.S. Biochemical Corp. |
| Plasmids | ||
| pGP159 | virA, virG, virB::lacZ of pTiA6 in IncP vector | 7 |
| pIB422 | pTiA6 virA virB::lacZ; IncP and pUC replion | 4 |
| pSM402 | virA, virG, virB::lacZ of pTiA6 in pVK102 | 28 |
| pSM243cd | virB::lacZ fusion of pTiA6 in pVK102 | 28 |
| pCH116 | virB::lacZ Plac-virG of pTiA6 in IncP vector | S. C. Winans (5) |
| pJZ105 | virA(I734N); IncW vector | S. C. Winans (6) |
| pPR1068 | pMAL-c2 derivative; NdeI at the start of MalE | New England Biolabs |
| pSL2L | pGC31; gbpR deletion at N terminus; nptII | 9 |
| pUFR047 | IncW broad-host-range vector | 12 |
| pWT120 | Ptac-chvE in pPR1068 | This work |
| pWT121 | Ptac-chvE lacIq in pUFR047 | This work |
| pWT122 | Ptac-chvE in pUFR047 | This work |