TABLE 3.
Effect of gbpR mutations on induction of virB::lacZ
Strain | β-Galactosidase (U) obtained with inducing compoundsa:
|
||
---|---|---|---|
HAP | HAP + arabinose | HAP + glucose | |
A136(pGP159) | 3.4 ± 0.2 | 388 ± 37 (113) | 48 ± 5.6 (14) |
A136gbpR(pGP159) | 4.4 ± 0.6 | 213 ± 11 (48) | 127 ± 1.4 (29) |
C58ΔvirA(pGP159) | 3.8 ± 0.2 | 348 ± 19 (90) | 102 ± 7.5 (26) |
C58ΔvirAgbpR(pGP159) | 5.3 ± 1.0 | 189 ± 0.9 (36) | 204 ± 6.0 (39) |
a Data are means ± standard errors of the means for three samples. The numbers in parentheses are the fold increases in induction calculated from the values with HAP alone. HAP was used at 250 μM. Arabinose and glucose were used at 10 mM.