TABLE 5.
Effect of ChvE level on induction of virB::lacZ
| Inducing compound | β-Galactosidase (U) obtained with straina:
|
Fold induction |
|---|---|---|
| C58ΔvirA(pGP159, pWT121) | ||
| HAP | 4.4 ± 1.5 | |
| + Arabinose | 167 ± 13 | 38 |
| + Glucose | 46 ± 4 | 10 |
| HAP + IPTG | 6.0 ± 1.4 | |
| + Arabinose | 278 ± 8 | 47 |
| + Glucose | 295 ± 18 | 50 |
a pWT121 directs the controlled high expression of chvE upon addition of IPTG. The data are means ± standard errors of the means for three independent samples. HAP was used at 250 μM. Arabinose and glucose were used at 10 mM. IPTG was used at 1 mM.