TABLE 6.
Responses of wild-type VirA and VirA(I734N) to induction by HAP with glucose and arabinose
Straina | β-Galactosidase (U) obtained with inducing compoundsb:
|
||
---|---|---|---|
HAP | HAP + arabinose | HAP + glucose | |
A136(pGP159) | 5.4 ± 0.2 | 388 ± 16 (72) | 27.4 ± 1.2 (5) |
A136(pCH116, pJZ105) | 10.6 ± 1.6 | 982 ± 34 (93) | 570 ± 7 (54) |
a pJZ105 contains VirA(I734N). pCH116 contains a Plac-virG promoter fusion to permit constitutive expression of virG and a virB::lacZ gene fusion.
b Data are means ± standard errors of the means for three samples. The numbers in parentheses are the fold increases in induction calculated from the values with HAP alone. HAP was used at 250 μM. Arabinose and glucose were used at 10 mM.