TABLE 7.
Induction of virB::lacZ in A. tumefaciens strains by specific phenolic compounds
| Inducing compoundsa | β-Galactosidase (U)b
|
|
|---|---|---|
| C58(pSM243cd) | KU12(pSM243cd) | |
| Acetosyringone | 109 ± 5 | 92 ± 1 |
| + Arabinose | 535 ± 8 | 145 ± 5 |
| + Glucose | 499 ± 22 | 133 ± 2 |
| Syringaldehyde | 42 ± 0.2 | 76 ± 2 |
| + Arabinose | 185 ± 6 | 107 ± 7 |
| + Glucose | 132 ± 3 | 92 ± 1 |
| Syringic acid | 9.1 ± 1.7 | 7.3 ± 1.6 |
| + Arabinose | 96 ± 5 | 126 ± 6 |
| + Glucose | 103 ± 1 | 92 ± 5 |
| Acetovanillone | 6.0 ± 1.5 | 6.9 ± 2.1 |
| + Arabinose | 192 ± 5 | 229 ± 5 |
| + Glucose | 163 ± 3 | 207 ± 2 |
| Ferulic acid | 14.7 ± 4 | 1.5 ± 0.1 |
| + Arabinose | 260 ± 4 | 242 ± 3 |
| + Glucose | 216 ± 4 | 211 ± 3 |
| 4-Hydroxyacetophenone | 2.7 ± 0.1 | 13.7 ± 0.1 |
| + Arabinose | 88 ± 5 | 371 ± 7 |
| + Glucose | 25 ± 1 | 354 ± 8 |
| p-Coumaric acid | 2.6 ± 0.5 | 7.1 ± 1.3 |
| + Arabinose | 54 ± 13 | 347 ± 19 |
| + Glucose | 5.7 ± 1.5 | 368 ± 8 |
| Phenol | 2.8 ± 0.1 | 3.8 ± 0.7 |
| + Arabinose | 30 ± 2 | 242 ± 15 |
| + Glucose | 5.0 ± 0.5 | 195 ± 12 |
a All phenolic compounds were used at 100 μM except for 4-hydroxyacetophenone, which was used at 250 μM. Arabinose and glucose were used at 10 mM.
b Data are means ± standard errors of the means for three samples. β-Galactosidase activities of cultures lacking phenolic compounds were less than 2 U.