TABLE 3.
Inhibition of the UV-induced increase of T4 2− EOP by derepression of the SOS system
| Strain | Relevant genotype | SOS-inducing pre-treatment of cells (54 J/m2)a | Relative EOP of T4 2−
|
|
|---|---|---|---|---|
| After pretreatment | After induction (135 J/m2)b | |||
| AB1157 | Wild-type | − | 1 | 220 |
| + | 2.1 | 19 | ||
| BT244 | lexA71::Tn5 | − | 1 | 2.5 |
| + | 4 | 12 | ||
| BT136 | lexA3 | − | 1 | 183 |
| + | 40 | 390 | ||
| BT346 | lexA3 recAo281 | − | 194 | |
| BT389 | lexA71::Tn5 ΔrecA | − | 7 | |
a The cells were irradiated (+) or not irradiated (−) with the indicated UV dose followed by 90 min of aeration at 30°C. About 10% of wild-type and lexA71::Tn5 cells survived the dose of 54 J/m2, while only about 0.0001% of the lexA3 cells survived.
b Cells with or without SOS-inducing pretreatment were irradiated with the indicated UV dose and aerated for 120 min at 30°C before determination of the EOP. The data are means from two or three independent experiments.