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. 2024 Jan 2;15:89. doi: 10.1038/s41467-023-44310-y

Fig. 3. NKTs expressing IL-12 acquire long-term longevity in vivo.

Fig. 3

a Schematic representation of the in vivo experiment in which non-tumor bearing NSG mice are infused with either GFP or IL12(I)GFP NKTs. b Representative flow cytometry plots (upper panel) and summary (lower panel) of the quantification of human NKTs (iNKT+CD45+) in peripheral blood samples collected 10, 20, and 30 days after GFP or IL12(i)GFP NKT infusion, and in livers and spleens at the time of euthanasia. Mean is shown; n = 9 mice, from two independent experiments; ****p < 0.0001; **p = 0.0023 in livers; **p = 0.0059 in spleens; two-way ANOVA with Bonferroni correction. Source data are provided as a Source Data file. c Representative flow cytometry plots (upper panel) and summary (lower panel) of the expression of CD62L in IL12(I)GFP NKTs at the time of euthanasia in the peripheral blood, livers and spleens. Mean is shown; n = 9 mice. Source data are provided as a Source Data file. d Genes differentially expressed in IL12(I)GFP and GFP NKTs expanded in vitro, and in IL12(I)GFP collected in vivo at the time of euthanasia (FDR < 0.05, top 35 genes); n = 3 donors for in vitro and n = 4 mice for in vivo. e Representative flow cytometry plots of 3 donors showing the expression of CD62L in NKTs overexpressing CD62L (CD62L.∆NGFR), expressing GFP with the knockout of CD62L (GFP KO.SELL) and in IL12(I)GFP and IL12(I)GFP NKTs with the knockout of CD62L (IL12(I)GFP KO.SELL) at day 14 of culture. f Representative flow cytometry plots of 4 mice showing the quantification of human NKTs (iNKT+CD45+) in peripheral blood samples collected 10, 20 and 30 days after IL12(i)GFP KO.SELL or CD62L.∆NGFR NKTs infusion, and in livers and spleens at the time of euthanasia. g Representative flow cytometry plots of 4 mice showing the expression of CD62L in IL12(I)GFP KO.SELL NKTs at the sacrifice in peripheral blood, livers and spleens.