Skip to main content
. 1998 Nov;180(21):5652–5659. doi: 10.1128/jb.180.21.5652-5659.1998

FIG. 6.

FIG. 6

(A) Protection of GST-GT from trypsin hydrolysis by moenomycin. Lanes: 1, standard molecular mass markers; 2, no trypsin; 3 and 7, trypsin/GST-GT ratio of 1:1,500 (wt/wt); 4 and 8, trypsin/GST-GT ratio of 1:150 (wt/wt); 5 and 9, trypsin/GST-GT ratio of 1:75 (wt/wt); 6 and 10, trypsin/GST-GT ratio of 1:15 (wt/wt). Lanes 7 to 10, hydrolysis in the presence of 5 mM moenomycin. Numbers at the left indicate sizes of standard molecular mass markers. (B) Trypsin digestion of PBP 2x. Lanes: 1, molecular mass standards; 2, no trypsin; 3 and 5, trypsin/PBP 2x ratio of 1:100 (wt/wt) 4 and 6, trypsin/PBP 2x ratio of 1:10 (wt/wt). Lanes 5 and 6, hydrolysis in the presence of 5 mM moenomycin.