TABLE 1.

Kinetic parameters of GST-PBP 1a*, PBP 1a*, and TP by using antibiotics and two substrate analogues^a

Protein	Cefotaxime k2/K (M−1 s−1)	Benzylpenicillin		Substrate analogue kcat/Km (M−1 s−1)
		k2/K (M−1 s−1)	k3 (s−1)	S2d	S2a
GST-PBP 1a*	25,840 ± 2,008	70,054 ± 3,923	1.3 × 10−5 ± 1 × 10−6	415 ± 39	32.8 ± 4
PBP 1a*	11,933 ± 2,309	34,200 ± 1,555	1.0 × 10−5 ± 1 × 10−6	128 ± 12	5.7 ± 0.4
TP	18,110 ± 1,173	47,700 ± 424	1.2 × 10−5 ± 1 × 10−6	195 ± 27	9.1 ± 1.6

^aValues are means ± standard deviations.