TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Strains | ||
| A348 | C58 chromosome, pTiA6NC (octopine-type) Ti plasmid | 37 |
| R10 | Wild-type strain with octopine-type Ti plasmid pTiR10 | 37 |
| KYC55 | R10, cured of pTiR10, arcB::Tn5gusA7 Knr | 5 |
| VIK10 | R10 (virG::pVIK119) virG-lacZ virG+ Knr | 25 |
| VIK11 | R10 (virG::pVIK123) virG-lacZ virG Knr | 25 |
| VIK12 | R10 (orf1::pVIK212) orf1-lacZ orf1 Knr | This work |
| VIK13 | R10 (virM::pVIK214) virM-lacZ virM Knr | This work |
| VIK14 | R10 (virL::pVIK127) virL-lacZ virL Knr | This work |
| VIK15 | R10 (orf4::pVIK178) orf4-lacZ orf4 Knr | This work |
| VIK16 | R10 (orf5::pVIK150) orf5-lacZ orf5 Knr | This work |
| VIK17 | R10 (orf6::pVIK151) orf6-lacZ orf6 Knr | This work |
| VIK18 | R10 (orf7::pVIK180) orf7-lacZ orf7 Knr | This work |
| VIK19 | R10 (virK::pVIK152) virK-lacZ virK Knr | This work |
| VIK20 | A348 (orf1::pVIK212) orf1-lacZ orf1 Knr | This work |
| VIK21 | A348 (virM::pVIK214) virM-lacZ virM Knr | This work |
| VIK22 | A348 (virL::pVIK127) virL-lacZ virL Knr | This work |
| VIK23 | A348 (orf4::pVIK178) orf4-lacZ orf4 Knr | This work |
| VIK24 | A348 (orf5::pVIK150) orf5-lacZ orf5 Knr | This work |
| VIK25 | A348 (orf6::pVIK151) orf6-lacZ orf6 Knr | This work |
| VIK26 | A348 (orf7::pVIK180) orf7-lacZ orf7 Knr | This work |
| VIK27 | A348 (virK::pVIK152) virK-lacZ virK Knr | This work |
| VIK28 | R10 virH virK Specr | This work |
| pVIK107 | Suicide vector for lacZ translational fusions | 25 |
| pVIK111 | Suicide vector for lacZ translational fusions | 25 |
| pVIK119 | 5′ end of virG (including promoter) in pVIK111 | 25 |
| pVIK123 | virG internal PCR fragment in pVIK111 | 25 |
| pSW219 | Plasmid containing virA and a virB::lacZ fusion | 4 |
| pVK219 | Cosmid containing vir region from pTiA6 | 27 |
| pWM91 | Suicide vector | 31 |
| pCC130 | BamHI fragment 15 from pTiA6NC cloned into pTZ18R | This work |
| pVIK127 | virL internal PCR fragment (codons 83–123) cloned into pVIK107 | This work |
| pVIK150 | orf5 internal PCR fragment (codons 107–256) cloned into pVIK107 | This work |
| pVIK151 | orf6 internal PCR fragment (codons 60–209) cloned into pVIK107 | This work |
| pVIK152 | virK internal PCR fragment (codons 54–127) cloned into pVIK107 | This work |
| pVIK178 | orf4 internal PCR fragment (codons 20–140) cloned into pVIK107 | This work |
| pVIK180 | orf7 internal PCR fragment (codons 7–129) cloned into pVIK107 | This work |
| pVIK182 | EcoRI fragment 11 of pTiC58 cloned into pTZ18R | This work |
| pVIK187 | BamHI fragment 7 of pTiC58 cloned into pTZ18R | This work |
| pVIK193 | 7.3-kb BamHI-NheI fragment from pVK219 cloned into pUC12chl | This work |
| pVIK200 | Specr cassette from pFP45Ω replacing virH locus cloned into pVIK193 | This work |
| pVIK202 | 5.3-kb SalI fragment from pVIK200 cloned into pWM91 | This work |
| pVIK204 | virH1 cloned into pUC19 | This work |
| pVIK206 | virH2 cloned into pUC19 | This work |
| pVIK212 | orf1 internal PCR fragment (codons 18–83) cloned into pVIK107 | This work |
| pVIK214 | virM internal PCR fragment (codons 24–103) cloned into pVIK107 | This work |