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. 2023 May 5;194(1):209–228. doi: 10.1093/plphys/kiad228

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© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Overproduced EXPA1 localizes to the CW. Z-stack projections of EXPA1:mCherry fluorescence in pRPS5A>GR>EXPA1:mCherry seedlings induced by Dex on solid MS medium for 7d in the primary root (A) and its xz optical cross-sections through RAM (B), TZ (C), and EZ (D). E, F)pRPS5A>GR>EXPA1:mCherry seedlings induced by Dex in liquid MS medium for 24 h; EZ of the primary root (E) and lateral root (F). G, H)pRPS5A>GR>EXPA1:mCherry seedlings induced as in (A) and after 10 min-treatment of the primary root with 550 mM sorbitol. Fluorescence channel (G) and its overlay with transmitted light (H). Scale bars correspond to 20 μm except in (A) (50 μm), (F) (5 μm), and (G & H) (10 μm).