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. 2023 May 5;194(1):209–228. doi: 10.1093/plphys/kiad228

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© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — EXPA1 overexpression reduces root growth by shortening the RAM. A) 7-d-old Arabidopsis seedlings of WT (top row) and pRPS5A>GR>EXPA1 line 8-4 (bottom row) grown on MS media pH4 supplemented either with DMSO (-Dex) or dexamethasone (+Dex). The scale bar is 5 mm. B) Length of roots, (C) the size of RAM + TZ, (D) the total number of cells (No. cells) in RAM + TZ, (E) the RAM size/cell number ratio, (F) No. of cells in RAM, and (G) No. of cells in TZ of 2 independent EXPA1 overexpressing lines (8-4 and 5-4) grown on MS media +/− Dex pH 5.8 and pH 4 relative to WT. Each experiment was repeated at least 3 times with a minimum of 10 seedlings in each category (n ≥ 30), and the error bars represent a 95% confidence interval. For all experiments, mixed model ANOVA using random effects for the different experiments was used with the Tukey test as a post hoc test. Statistically significant differences within genotypes and treatments at alpha 0.05 (*) and 0.001 (***) are shown.