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TAP-N Medium for gametogenesis:
| Reagent | Final concentration | Amount |
|---|---|---|
| H2O | N/A | 850 mL |
| Tris base (H2NC(CH2OH)3) | 20 mM | 2.42 g |
| TAP-N salts | N/A | 25 mL |
| Phosphate solution | N/A | 1 mL |
| Hutner’s trace elements | N/A | 1 mL |
| Acetic acid, glacial | to pH 7 | ∼1.8 mL |
| H2O | N/A | to 1 L |
| Total | N/A | 1 L |
Add components in order and mix completely between additions. Autoclave solution for 20 min at 140°C and store at RT. The medium is stable for at least one year.
