The final reactions should consist of:
| Reagent | Amount |
|---|---|
| DNA template (cell extract) | 0.5 μL |
| Platinum 2 HS Master Mix | 3 μL |
| Primer 1 (20 mM) | 0.06 μL |
| Primer 2 (20 mM) | 0.06 μL |
| 2 M Betaine | 2.32 μL |
| EvaGreen (Plus) 20× in Water | 0.06 μL |
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