REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli DH5α	NEB	Cat#C2987H
E. coli GB06	Lab stock	N/A
E. coli DB3.1	Lab stock	N/A
Chemicals, peptides, and recombinant proteins
K2HPO4	Sigma-Aldrich	P3786
KH2PO4	Merck	1.04873
MgSO4·7H2O	Sigma-Aldrich	3138
NH4Cl	Sigma-Aldrich	A9434
CaCl2·2H2O	Merck	C7902
KCl	Merck	1.04936
Tris base	Sigma-Aldrich	T1503
Acetic acid, glacial	Merck	1.00063
Agar	Fisher Scientific	BD 214010
Sucrose	Alfa Aesar	36508
Alt-R S.p. Cas9 Nuclease V3	IDT	Cat#1081058
Platinum SuperFi II PCR master mix (2×)	Thermo Fisher Scientific	Cat#12368010
Platinum II Hot-Start PCR master mix (2×)	Thermo Fisher Scientific	Cat#14000013
Paromomycin	TCI	P2092
Nourseothricin	Jena Bioscience	AB-102XL
Blasticidin S	Carl Roth	CP14.2
Spectinomycin	Merck	S4014-5G
EvaGreen Plus	Biotium	31077-T
GelGreen	Biotium	41005
Betaine	Merck	61962
NEBuilder HiFi assembly master mix	NEB	E2621L
BspQI	NEB	R0712L
BtsI-v2	NEB	R0667L
SacI-HF	NEB	R3156L
EcoRI-HF	NEB	R3101L
NheI-HF	NEB	R3131S
Hutner’s trace elements	Chlamydomonas Resource Center	N/A
IGEPAL CA-630	Merck	I8896
Difco Bacto Agar	BD	BD 214010
Nuclease-free duplex buffer	IDT	11-05-01-12
QuickExtract solution	Lucigen	QE09050
Critical commercial assays
ZymoPURE II Plasmid Midiprep Kit	Zymo Research	D4200
ZymoPURE II Plasmid Miniprep Kit	Zymo Research	D4211
DNA Clean & Concentrator-25	Zymo Research	D4033
Neon electroporator	Thermo Fisher Scientific	MPK5000
10 μL Neon transfection kit	Thermo Fisher Scientific	MPK1025
QuantStudio 7 Pro	Thermo Fisher Scientific	A43185
Light board	Artograph	LightPad 940LX
FACS sorter	Sony	MA-900
Experimental models: Organisms/strains
Chlamydomonas WT (+)	Chlamydomonas Resource Center	CC-620
Chlamydomonas WT (-)	Chlamydomonas Resource Center	CC-621
Chlamydomonas WT (-)	Dutcher Lab	CC124 32M
Chlamydomonas WT (+)	Chlamydomonas Resource Center	CC-125
Oligonucleotides
Alt-R CRISPR-Cas9 tracrRNA, 20 nmol	IDT	Cat#1072533
CRISPR-Cas9 crRNAs, 2 nmol	IDT	N/A
crMTM_q_1F	IDT	TGCTAGCTCAATCTGACTGAAGGT
crMTM_q_1R	IDT	GGGACATGGTGCTCGGGG
crMTP_q_1F	IDT	AGTATCACAGTACGGAATGCCC
crMTP_q_1R	IDT	TCATTGTTTGCTAGGGGTGC
Recombinant DNA
pAB262_NAT-R	This study	N/A
pAPN_BSD	This study	N/A
pAPN_AphVIII	This study	N/A
pAPN_AadA	This study	N/A
pAPN_noAB-ARMC2-BSD-ccdb	This study	N/A
pAPN_noAB-ARMC2-PAR-ccdb	This study	N/A
pAPN_noAB-ARMC2-NAT-ccdb	This study	N/A
Custom synthetic fragments	Twist Bioscience	N/A
Custom synthetic fragments	IDT	N/A
Custom synthetic fragments	Eurofins Genomics	N/A
Software and algorithms
Geneious Prime	Biomatters	2021-2023.0.1
Design and Analysis	Thermo Fisher Scientific	2.6.0
Fiji	Fiji	https://imagej.net/software/fiji/
iQ3	Andor	https://andor.oxinst.com/products/iq-live-cell-imaging-software/