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Phosphate solution:
| Reagent | Final concentration | Amount |
|---|---|---|
| H2O | N/A | 80 mL |
| K2HPO4 | 1.65 M | 28.8 g |
| KH2PO4 | 1.06 M | 14.4 g |
| H2O | N/A | to 100 mL |
| Total | N/A | 100 mL |
Autoclave solution for 20 min at 140°C and store at 4°C. The solution is stable for at least 5 years if sealed properly.
