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TAP salts:
| Reagent | Final concentration | Amount |
|---|---|---|
| H2O | 800 mL | |
| NH4Cl | 280 mM | 15 g |
| MgSO4·7H2O | 16.2 mM | 4 g |
| CaCl2·2H2O | 13.6 mM | 2 g |
| H2O | to 1 L | |
| Total | N/A | 1 L |
Dissolve in 800 mL and adjust final volume to 1 L. Autoclave solution for 20 min at 140°C and store at 4°C. The solution is stable for at least 5 years.
