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. 2023 Dec 26;15(12):1093–1103. doi: 10.4252/wjsc.v15.i12.1093

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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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High glucose decreased the mitochondrial membrane potential level of human adipose tissue-derived mesenchymal stem cells. The tetramethylrhodamine ethyl ester (TMRE) fluorescence intensity values of human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) cultured in high glucose for 7 d were substantially declined compared to fluorescent intensity values of cells cultured in low glucose. Live fluorescent images showed a weak signal of TMRE stain in hAD-MSCs after being placed in high glucose for 7 d compared to strong TMRE signal in low glucose cultured cells. n = 4, ^aP < 0.05 compared to low glucose control. Fluroscnet images were captured at Texas Red filter using Cytation 5 (BioTek, United States). TMRE: Tetramethylrhodamine ethyl ester; RFU: Relative fluorescence units.