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. 2023 Dec 26;15(12):1093–1103. doi: 10.4252/wjsc.v15.i12.1093

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©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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The protein levels of mitochondrial complexes were reduced in high glucose cultured human adipose tissue-derived mesenchymal stem cells. Western blot protein analysis showed significant reduction in the protein levels of NDUF6B (complex I), COX5a (complex IV), and ATP5a (complex V) in high glucose cultured human adipose tissue-derived mesenchymal stem cells comparing to low glucose cultured cells, while the protein levels of SDBH (complex II) and UQCRC2 (complex III) were not significantly changed in both culturing conditions. n = 3, ^aP < 0.05 compared to low glucose control.