Table 2.

Accuracy in human CSF for sex steroid hormones.

		Spiked pg/mL	Baseline pg/mL	Measured pg/mL	Accuracy %
DHT	Low level	1.5	0	1.50	100
	High level	40	1.77	44.1	106
Testosterone	Low level	7.5	1.53	9.62	108
	High level	200	26.5	228	101
E1	Low level	0.6	0.82	1.31	83
	High level	16	2.42	17.9	97
E2	Low level	0.3	0	0.36	120
	High level	16	0	17.5	109
DHEA	Low level	7.5	38.4	44.9	87
	High level	400	91.8	456	91
4D	Low level	100	52.3	156	104
	High level	250	52.3	312	104
P	Low level	3.75	0	4.23	113
	High level	15	0	15.7	105

Accuracies were calculated as (measured level − baseline level)/spiked level × 100. Five replicates for each level evaluated were used for the accuracy determinations. Part of the validation data for testosterone have previously been published (16).

4D, androstenedione; DHEA, dehydroepiandrosterone; DHT, dihydrotestosterone; E1, estrone; E2, estradiol; P, progesterone.