TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pAD-GAL4 | Activation domain cloning vector; AmprLEU2 | Stratagene |
| pBD-GAL4 | Binding domain cloning vector; CmrTRP1 | Stratagene |
| p53 | Murine p53-binding domain fusion; AmprTRP1 | Stratagene |
| pSV40 | Simian virus 40 large-T-antigen–activation domain fusion; AmprLEU2 | Stratagene |
| pLaminC | Human lamin C-binding domain fusion; AmprTRP1 | Stratagene |
| pBD-Z1 | FrzZ first domain-binding domain fusion; CmrTRP1 | This study |
| pBD-Z2 | FrzZ second domain-binding domain fusion; CmrTRP1 | This study |
| pBD-E | FrzE-binding domain fusion; CmrTRP1 | This study |
| pBD-AsgA | AsgA-binding domain fusion; CmrTRP1 | Lynda Plamann, University of Missouri |
| pADLIB | Library of random fragments from M. xanthus DZF1-activation domain fusions; AmprLEU2 | This study |
| pAD-RpoE1 | RpoE1-activation domain fusion; AmprLEU2 | This study |
| pUC18 | General cloning vector; Ampr | 26 |
| pZErO-2 | Zero Background cloning vector; Kmr | Invitrogen |
| pZRpoE1 | Internal fragment of rpoE1 cloned in pZErO-2; Kmr | This study |
| pZOrf5 | Internal fragment of orf5 cloned in pZErO-2; Kmr | This study |
| pBS9 | 6,464-bp fragment containing the 3′ end of frzZ and downstream genes cloned in pBlueScript | This study |