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. 2023 Dec 20;17:1270195. doi: 10.3389/fncel.2023.1270195

FIGURE 4.

FIGURE 4

Effect of early social isolation (ESI) and different housing conditions on plasma corticosterone levels, Nr3c1 gene expression, and GR protein expression in the PFC of adolescent male (A,C,E) and female (B,D,F) rats. Plasma corticosterone data (A,B) are expressed as ng/ml of mean ± SEM (n = 5–6/group; 1 outlier in SH-ESI males and 1 outlier in SH-CTRL females). Gene expression data (C,D) represent 2–ΔΔCt values calculated by the ΔΔCt method. GR protein levels are shown as a ratio between nuclear and cytosolic fraction (E,F). Protein data are expressed as percentages vs. SH-CTRL male or female rats. Histograms represent the mean ± SEM (n = 5–6/group; 1 outlier in SH-CTRL females and 1 outlier in CN-CTRL females). Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparisons test (*p < 0.05, **p < 0.001, ****p < 0.0001). Representative immunoblots for GR protein levels (95 kDa) and β-Actin (43 kDa) are shown in panel (G).