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Southern blot analysis of two-dimensional gel of guaB heterozygote plasmid DNA. (A) B31-80.74 plasmid DNA was electrophoresed through 0.3% agarose before and after being soaked in chloroquine and rotated 90°. DNA was transferred to a nylon membrane and sequentially hybridized with probes derived from guaB (A) and then cp32 (orf12-13), lp16 (p5 [3]), and lp54 (ospA) (B). Marker sizes are in kilobases.
