FIG. 1.

Difference spectroscopy of membrane proteins isolated from microaerobically and aerobically grown A. brasilense Sp7. (A and C) Dithionite-reduced-minus-air-oxidized spectra between 400 and 700 nm (5 mg of protein/ml) (A) and between 500 and 700 nm (3 mg of protein/ml) (C). (B and D) CO plus dithionite-reduced-minus-dithionite-reduced difference spectra between 400 and 700 nm (2 mg of protein/ml) (B) and between 500 and 700 nm (2 mg of protein/ml) (D).