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(A) Preparation of a Misacylated Suppressor tRNACUA Transcript by T4 RNA Ligase-Mediated Attachment of an Aminoacylated pdCpA Derivative to an Abbreviated tRNA-COH Lacking the Dinucleotide Sequence Normally Found at the 3′-End of tRNAs. (B) Introduction of a Noncanonical Amino Acid into a Protein Synthesized Ribosomally by Suppression of Nonsense Codon UAG in the mRNA with a Misacylated tRNACUA. All of the Activated tRNAs in This Report Are Amber Suppressor tRNAs
