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Coupled in vitro transcription/translation guided by (A) template 1 (DHFR, Ala9stop10) or (B) template 2 (DHFR Pro9stop10) in the presence of different activated suppressor tRNAs. Analysis was performed by SDS-PAGE and autoradiography. Yields are expressed as a percentage of those obtained in the presence of yeast suppressor tRNAPhe activated with phenylalanine. A parallel control sample in the absence of any suppressor tRNA (denoted as C) was run for each experiment.
