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Effect of E. coli EF-P on in vitro translation efficiency from (A) template 1 (DHFR, Ala9stop10) or (B) template 2 (DHFR, Pro9stop10) in the presence of suppressor tRNAHyb acylated with proline (Pro-tRNAHyb). Yields (%) are presented comparatively to the DHFR yield in the presence of yeast suppressor tRNAPhe aminoacylated with phenylalanine (Phe-tRNAPhe) without the addition of exogenous EF-P.
