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. Author manuscript; available in PMC: 2024 Jan 3.
Published in final edited form as: J Am Chem Soc. 2023 Oct 23;145(43):23600–23608. doi: 10.1021/jacs.3c07524

Figure 5.

Figure 5.

Effect of E. coli EF-P (1.75 μM) on in vitro translation efficiency from (A) template1 (DHFR, Ala9stop10) or (B) template 2 (DHFR, Pro9stop10) in the presence of suppressor tRNAHyb aminoacylated with proline (Pro-tRNAHyb), phenylalanine (Phe-tRNAHyb), or lysine (Lys-tRNAHyb). The outcome of the experiments was monitored by SDS PAGE and autoradiography. Yields (%) are presented comparatively for each amino acid. Control samples (denoted as C) were run in the absence of any suppressor tRNA without (−) or with (+) exogenous EF-P.