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Effect of E. coli EF-P (1.75 μM) on in vitro translation efficiency from (A) template 1 (DHFR, Ala9stop10) or (B) template 2 (DHFR, Pro9stop10) in the presence of suppressor tRNAHyb, activated with proline, or compound 1, 2, or 3. The outcome of the experiment was analyzed by SDS-PAGE and autoradiography. Yields (%) are presented comparatively for each compound.
