TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pAD-GAL4 | Activation domain cloning vector; AmprLEU2 | Stratagene |
| pBD-GAL4 | Binding domain cloning vector; CmrTRP1 | Stratagene |
| p53 | Murine p53-binding domain fusion; AmprTRP1 | Stratagene |
| pSV40 | Simian virus 40 large-T-antigen–activation domain fusion; AmprLEU2 | Stratagene |
| pLaminC | Human lamin C-binding domain fusion; AmprTRP1 | Stratagene |
| pBDZ1 | FrzZ first domain-binding domain fusion; CmrTRP1 | This study |
| pBDZ2 | FrzZ second domain-binding domain fusion; CmrTRP1 | This study |
| pBDE | FrzE-binding domain fusion; CmrTRP1 | This study |
| pBD-AsgA | AsgA-binding domain fusion; CmrTRP1 | Lynda Plamann, Univer-sity of Missouri |
| pADLIB | Library of random fragments from M. xanthus DZF1-activation domain fusions; AmprLEU2 | This study |
| pADZ165a | C-terminal AbcA-activation domain fusion; AmprLEU2 | This study |
| pUC18 | General cloning vector; Ampr | 41 |
| pZErO-2 | Zero Background cloning vector; Kmr | Invitrogen |
| pZABC | Internal fragment of abcA cloned in pZErO-2; Kmr | This study |
| pZORF3 | Internal fragment of orf3 cloned in pZErO-2; Kmr | This study |
| pZCTR | Internal fragment of ctrA cloned in pZErO-2; Kmr | This study |
| pABCS3.5 | 3.5-kb SacI fragment of abc operon cloned in pUC18; Ampr | This study |
| pABCS1.8 | 1.8-kb SacI fragment of abc operon cloned in pUC18; Ampr | This study |
| pABCS1.3 | 1.3-kb SacI fragment of abc operon cloned in pUC18; Ampr | This study |
| pABCBK2.1 | 2.1-kb BamHI-KpnI fragment of abc operon cloned in pUC18; Ampr | This study |