TABLE 3.
Two-hybrid reporter gene assays
| Plasmid combination
|
Reporter gene assays resulta
|
||
|---|---|---|---|
| Bait | Test | SD-Leu-Trp-His | β-Gal |
| pBDZ1 | pADZ165a | ++ | + (11 hb) |
| p53 | pADZ165a | + | − |
| pBDZ2 | pADZ165a | + | − |
| pBDE | pADZ165a | + | − |
| pBDAsgA | pADZ165a | + | − |
| p53 | pSV40 | +++ | + (5 h) |
a
Various bait fusions (Table 2) cloned in pGAL4-BD were screened for reporter gene activity when transformed into S. cerevisiae YRG-2 in combination with the test activation domain fusion expressed from pADZ165a. The control combination p53+pSV40 was used in both assays.
b
Time when blue color was first observed on filters.