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. 2024 Jan 2;24:4. doi: 10.1186/s12935-023-03181-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — YTHDF1 improves lncRNA-FOXD1-AS1 mRNA stability in a m⁶A-dependent manner. A The binding of lncRNA-FOXD1-AS1 to the YTHDF1 protein in PC was confirmed by RIP-qPCR. B YTHDF1 was assessed via Western blotting in PC using cell lysate, full-length biotinylated lncRNA-FOXD1-AS1, and only beads (NC). C Western blot analysis of YTHDF1 in PC with cell lysate (Ly.), completely biotinylated-lncRNA-FOXD1-AS1 (#1), lncRNA-FOXD1-AS1(0–400) with m⁶A pattern site 1 and 2-WT (#2) and site 1 and 2-MUT (#3), lncRNA-FOXD1-AS1(400–600) with m⁶A pattern site 3-WT (#4) and site 3-MUT (#5), and beads alone (NC). D Analysis of lncRNA-FOXD1-AS1 levels with ACTIN using qRT-PCR in PC cells treated with actinomycin D (10 μM)