Table 1.
Primers used in this study
| Primers | Primer sequence (5′–3′) | Function |
|---|---|---|
| pUC118-CTJ1-F/R |
F:TATGACCATGATTACGAATTCGCCCAATTTCCCCAGTGTTATCCTGTG R:CAGGTCGACTCTAGAGGATCCTCTGAGGGAGGGAAGAAGAGT |
To amplify the CTJ sequence for analysis of the progeny genome DR motif |
| pUC118-CTJs-F/R |
F:ATGACCATGATTACGAATTCTGGTGTATTGCCAATCCTTTGCCGA R:AGGTCGACTCTAGAGGATCCGGGCGTGAAATACAAACTTGAGTTT |
To construct the standard pUC118-CTJ for TaqMan dual quantification PCR |
| pUC118-UL31 s-F/R |
F:TATGACCATGATTACGAATTCATTCAATTTCGCTCGTCTCA R:CAGGTCGACTCTAGAGGATCCCTTGTACCCAGGGATGCCAC |
To construct the standard pUC118-UL31 for TaqMan dual quantification PCR |
| pTerS-F/R |
F:CAATCCAGATGCATAGTCCGCGTG R:GAGACGGGACTCCGAAGGTAGT |
To amplify S-terminus probe for southern blot |
| Qpcr-CTJ-F/R |
F:GGTGGAGTTGGCATGTTG R:CTTCCATAGCAGTGCATTGA |
To amplify CTJ sequence for TaqMan dual quantification PCR |
| Qpcr-UL31-F/R |
F:CCATGAGAGCCAGATCTTC R:CTCCCGTACTATGGCTAAC |
To amplify UL31 gene for TaqMan dual quantification PCR |